Nanoengineering of eco-friendly silver nanoparticles using five different plant extracts and development of cost-effective phenol nanosensor

The production of environmentally friendly silver nanoparticles (AgNPs) has aroused the interest of the scientific community due to their wide applications mainly in the field of environmental pollution detection and water quality monitoring. Here, for the first time, five plant leaf extracts were used for the synthesis of AgNPs such as Basil, Geranium, Eucalyptus, Melia, and Ruta by a simple and eco-friendly method.

  • Stable AgNPs were obtained by adding a silver nitrate (AgNO3) solution with the leaves extract as reducers, stabilizers and cappers. Only, within ten minutes of reaction, the yellow mixture changed to brown due to the reduction of Ag+ ions to Ag atoms. The optical, structural, and morphology characteristics of synthesized AgNPs were determined using a full technique like UV-visible spectroscopy, FTIR spectrum, XRD, EDX spectroscopy, and the SEM. Thus, Melia azedarach was found to exhibit smaller nanoparticles (AgNPs-M), which would be interesting for electrochemical application.
  • So, a highly sensitive electrochemical sensor based on AgNPs-M modified GCE for phenol determination in water samples was developed, indicating that the AgNPs-M displayed good electrocatalytic activity. The developed sensor showed good sensing performances: a high sensitivity, a low LOD of 0.42 µM and good stability with a lifetime of about one month, as well as a good selectivity towards BPA and CC (with a deviation less than 10%) especially for nanoplastics analysis in the water contained in plastics bottles.
  • The obtained results are repeatable and reproducible with RSDs of 5.49% and 3.18% respectively. Besides, our developed sensor was successfully applied for the determination of 1000 ml plastic bottle phenol in tap and mineral water samples. The proposed new approach is highly recommended to develop a simple, cost effective, ecofriendly, and highly sensitive sensor for the electrochemical detection of phenol which can further broaden the applications of green silver NPs.

First Report of Black Spot Caused by Penicillium citreosulfuratum on Saffron in Chongming Island, China.

  • Saffron (Crocus sativus L.) is a world-famous source of dye and spices and an important medicinal plant, which is cultivated on a large scale on Chongming Island (31.62° N, 121.39° E) in Shanghai, China. In August 2020, a survey of saffron was conducted in this area, and black spots were observed on about 10% of plants.
  • Characteristics of the disease were: The bottom of the corm was darkened in the groove and scattered black spots could be observed after peeling off the membranous scale leaves. The junction of lesions and healthy parts was light brown. As the lesions expanded, approximately 80% of the surface of the corm became dark brown to bluish gray.
  • The inside of the corm was also necrotic. In order to isolate the pathogen, ten diseased corms with typical symptoms were selected. All corms were first treated with 75% ethanol for 30 s, 0.1% HgCl2 for 5 min, and then rinsed with sterile water 5 times. Next, tissue pieces (5 mm × 5 mm) at the margin of lesions were cut out and placed on the potato dextrose agar (PDA) medium. After incubating at 28°C for 5 days, fungi were separated and purified by using the hyphal-tip technique.
  • A total of six pure cultures with different colony morphologies were obtained, of which only the isolate MF3 was present in ten diseased corms. The isolate MF3 was inoculated on the PDA and cultured at 28°C for 10 days and characteristics of the fungus colonies were: colonies sub-circular, reaching 28 to 30 mm diam, from above rough, dense, fluffy, blue-gray, with some white spots and central point raised, and outer margin form an irregular, narrow, white ring; from below, yellow with light-green. The hyphae were slender, with many septa.
  • The conidiophores were typically smooth walled, short, and slender and either monoverticillate with very short stipes or as irregularly biverticillate. Phialides were ampulliform, 5 to 10 per metula, 5 to 8 × 2 to 3 µm. Conidia were smooth and globose, and ranged in diameter from 1.4 to 1.7 µm (n=50).
  • Molecular identification of the fungus was made by PCR amplification of the internal transcribed spacer (ITS) region of rDNA and β-tubulin gene using primers ITS1/ITS4 (White et al. 1990), TUB2Fd/TUB4Rd (Aveskamp et al. 2009) respectively.
  • The ITS (MW881446) and β-tubulin (MW911464) sequences of the fungus were similar to the ITS (MN592912) and β-tubulin (KY469126) sequences of the epitype of Penicillium citreosulfuratum with 99.81% and 99.56% identity, respectively.
  • According to the morphological and molecular characterization, the isolate MF3 was identified as P. citreosulfuratum (Visagie et al. 2016). For pathogenicity testing, the fungus was grown on PDA and incubated at 28°C for 5 days.
  • Then mycelial plugs (5 mm diam.) were inoculated on the scalpel incision square wounds of surface-disinfected corms and mock-inoculated corms received only PDA plugs. Corms were placed in sterile plastic bottles and observed after culturing at 28°C for 21 days. Each treatment had three replicates and the experiment was repeated twice.
  • The results showed that corms inoculated with P. citreosulfuratum developed diseased with similar symptoms as in the field. No disease symptoms were observed on control corms. Re-isolations were performed from inoculated corms, and all re-isolated fungi were confirmed as P. citreosulfuratum, verifying the fungus as the pathogen based on Koch’s postulates.
  • To our knowledge, this is the first report of the pathogen causing black spot disease of saffron. Although the disease is not fatal to saffron, to a certain extent it will cause a reduction in the production of the crop. In addition, this pathogen has not been reported to be pathogentic to other plant species.
2sars
2sars

 

First Report of Spot Blotch Caused by Bipolaris sorokiniana on Winter Rye in Kentucky.

The Kentucky distilling industry ranks as one of the state’s largest industries and continues to expand. In 2017, the Kentucky distilling industry was responsible for approximately $235 million in state and local tax revenues (Coomes and Kornstein, 2019). Rye (Secale cereale L.) grains are a vital component for production of some distilled spirits.

  1. Although winter rye is produced on relatively few hectares in Kentucky currently, a recent initiative has supported expanding production to help meet the growing demand of local distilleries.
  2. University of Kentucky winter rye research field trials were visited in Caldwell and Logan Counties, KY in May 2018, and in Fayette County, KY in May 2019. Leaves were collected that had dark brown, oval to irregular-shaped lesions with definite margins and yellow halos. Symptoms were present on approximately 50% to 80% of the flag leaves, with severity ranging from 5% to 30% of the flag leaf area affected.
  3. Leaves were surface-disinfested by soaking in a 2% NaOCl solution for 1 min and rinsed twice in sterilized water and then placed in a humidity chamber (plastic bag with moist paper towels) at room temperature (approximately 24°C) to induce fungal sporulation.
  4. Seventeen single-spore isolates were obtained and stored at -80°C in 15% glycerol solution. Isolates were grown on potato dextrose agar under 12 h cycles of white light/darkness for 5 days. Colonies were gray to black. Conidia that formed were mostly straight or slightly curved, dark olivaceous brown, 3-7 septate, and 41.0-90.4 × 15.2-29.3 µm.
  5. Based on the symptoms observed on the collected leaves and these morphological characteristics similar to those described by Chang and Hwang (2000) and Manamgoda et al. (2014), the fungus was tentatively identified as Bipolaris sorokiniana (Sorokin) Shoemaker. The sequence of internal transcribed spacer regions (ITS) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were used to identify three isolates (18Bs004, 18Bs111 and 19Bs064) using primer ITS1/ITS4 (White et al. 1990) and GPD1/GPD2 (Berbee et al. 1999), respectively.
  6. The sequences were deposited in GenBank with accession numbers MT457817, MT457818 and MZ066635 for ITS sequences and MZ073644 to MZ073646 for GAPDH sequences. BLAST searches with ITS and GAPDH sequences matched 100% identity (344/344 bp and 515/515 bp for ITS and GAPDH sequences, respectively) to B. sorokiniana (GenBank accession No. MT254731 and MH844813, respectively).
  7. To prove pathogenicity, a conidial suspension (1 × 105 conidia/ml) was used to inoculate 15-day-old cultivar ‘Serafino’ winter rye plants in the greenhouse. Leaves of 8 plants were inoculated with 50 ml of the conidial suspension using a spray bottle. Plants were covered with a transparent plastic bag for 48 h, and symptoms were observed 10 days after inoculation.
  8. Leaf lesions, similar to those described above, were present on all inoculated plants, but no symptoms were observed on non-inoculated control plants. Bipolaris sorokiniana was reisolated from symptomatic leaves and the identity of the pathogen was confirmed based on the morphology previously described.
  9. To our knowledge, this is the first report of spot blotch caused by B. sorokiniana on winter rye in Kentucky, but B. sorokiniana has been reported on rye in the neighboring state of Virginia (Roane 2009).
  10. Kentucky produces approximately 150,000 and 4,000 ha of winter wheat (Triticum aestivum) and winter barley (Hordeum vulgare) annually, respectively, which are both known hosts of B. sorokiniana (Kumar et al. 2002).
  11. An isolate of B. sorokiniana from rye was reported by Ghazvini and Tekauz (2007) to be less virulent on barley differential lines.

Flugs® - Plastic Fly Bottles

49-100 Genesee Scientific 1000 Flugs/Unit

Droso-Plugs®, Plastic Bottles

59-194P Genesee Scientific 2560 Plugs/Unit

Duran Bottles 100Ml Plastic coat - PK10

F94003 Scientific Laboratory Supplies PK10

Plastic bottles 250ml supply/waste - PK2

WAT4842 Scientific Laboratory Supplies PK2

Flystuff (59-194P) Droso-Plugs Cap for Plastic Bottles - PK2560

FLY1094 Scientific Laboratory Supplies PK2560

Disposable Meat Sample Plastic tubes with caps (5 ml) (1000/pk)

MST5-1000 Alpha Diagnostics 1 pk

Corning 1L Square Polycarbonate Storage Bottles with 45mm Caps - PK24

431433 Scientific Laboratory Supplies PK24

Corning 150mL Square Polycarbonate Storage Bottles with 45mm Caps - PK24

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Corning 150mL Easy Grip Polystyrene Storage Bottles with 45mm Caps - PK24

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Costar 1L Traditional Style Polystyrene Storage Bottles with 45mm Caps - PK12

8396 Scientific Laboratory Supplies PK12

Costar 125mL Traditional Style Polystyrene Storage Bottles with 45mm Caps - PK24

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Disposable Meat Sample Plastic tubes with caps (5 ml) (250/pk)

MST5-250 Alpha Diagnostics 1 pk

Reagent Bottle Clear Narrow neck with plastic stopper 100ml - PK10

BOT2136 Scientific Laboratory Supplies PK10

Reagent Bottle Clear Narrow neck with plastic stopper 250ml - PK10

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Reagent Bottle Clear Narrow neck with plastic stopper 50ml - PK10

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Reagent Bottle Amber Narrow neck with plastic stopper 500ml - PK10

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Reagent Bottle Amber Narrow neck with plastic stopper 50ml - PK10

BOT2156 Scientific Laboratory Supplies PK10

SCREW CAPS WITH "O" RINGS. BROWN

SCO-BR CORNING 500/pk

DURAN protect Laboratory bottle with DIN thread plastic coated GL 25 without cap and without pouring ring 10 ml - PK10

BOT1190 Scientific Laboratory Supplies PK10

Pyrex WM Bottles with Cap and Ring - 250mL - PK10

152306 Scientific Laboratory Supplies PK10

LCP EZ-Cut films (50 pieces, with liners)

MLCP-EZCTF-50 MiTeGen 50 FILMS

Bottle Duran Plastic Coat 1L W/out Cap - EACH

2180554 Scientific Laboratory Supplies EACH

Bottles 2L Nalgene with Quick Connect

BTLN52 Next Advance 2pack

Disposable Sterile Media Bottles - 50 ml

PW1277-1X100NO EWC Diagnostics 1 unit

Disposable Sterile Media Bottles - 50 ml

PW1277-1X50NO EWC Diagnostics 1 unit

Pump Sampler Set with Glass Bottles - EACH

SAM1517 Scientific Laboratory Supplies EACH

Bottle Duran Plastic Coated 5L. - EACH

2180573 Scientific Laboratory Supplies EACH

Bottles Cf 250 Ml Pc +Cap - PK4

3140-0250 Scientific Laboratory Supplies PK4

Bottle Azlon PPR Brown 150ml - PK10

BOT3382B Scientific Laboratory Supplies PK10

Bottle Pyrex Plastic Coat 10Ltr - EACH

1518/18 Scientific Laboratory Supplies EACH

Reagent Bottle Plastic Coated 2L - PK10

151812 Scientific Laboratory Supplies PK10

Plastic Bottle, Wide Neck, 125ml, Natural

B003N-125 Bio Basic 10, 10UNIT

Plastic Bottle, Wide Neck, 15ml, Natural

B003N-15 Bio Basic 10, 10UNIT

Plastic Bottle, Wide Neck, 250ml, Natural

B003N-250 Bio Basic 10, 10UNIT

Plastic Bottle, Wide Neck, 30ml, Natural

B003N-30 Bio Basic 10, 10UNIT

Plastic Bottle, Wide Neck, 500ml, Natural

B003N-500 Bio Basic 5UNIT

Plastic Bottle, Wide Neck, 70ml, Natural

B003N-70 Bio Basic 10, 10UNIT

Pump Sampler Set with 12 x PE Bottles 150ml - EACH

SAM1521 Scientific Laboratory Supplies EACH

Pump Sampler Set with 5 x PE Bottles 250ml - EACH

SAM1522 Scientific Laboratory Supplies EACH

100ml Clear PET Plastic Sirop Bottle - EACH

100MLCPET Scientific Laboratory Supplies EACH

DURAN protect Laboratory bottle plastic coated GL 45 10000 ml - EACH

BOT1236 Scientific Laboratory Supplies EACH

DURAN protect Laboratory bottle plastic coated GL 45 15000 ml - EACH

BOT1238 Scientific Laboratory Supplies EACH

DURAN protect Laboratory bottle plastic coated GL 45 20000 ml - EACH

BOT1240 Scientific Laboratory Supplies EACH

Plastic Bottle, Wide Neck, Sterile, 125ml, Natural

B003N-S125 Bio Basic 10, 10UNIT

Plastic Bottle, Wide Neck, Sterile, 15ml, Natural

B003N-S15 Bio Basic 10, 10UNIT

Plastic Bottle, Wide Neck, Sterile, 250ml, Natural

B003N-S250 Bio Basic 10, 10UNIT

Plastic Bottle, Wide Neck, Sterile, 30ml, Natural

B003N-S30 Bio Basic 10, 10UNIT

Plastic Bottle, Wide Neck, Sterile, 500ml, Natural

B003N-S500 Bio Basic 5UNIT

Plastic Bottle, Wide Neck, Sterile, 70ml, Natural

B003N-S70 Bio Basic 10, 10UNIT

White Mob Caps in Plastic Bags - PK100

SAF9580 Scientific Laboratory Supplies PK100

MiniVac Plastic Tubing Instrument to Bottle Lid - EACH

ARG1242 Scientific Laboratory Supplies EACH

Media bottles 500 ml petg - PK70

Z377058-1CS Scientific Laboratory Supplies PK70

Duran Bottles 1000ml No Cap - PK10

F93990 Scientific Laboratory Supplies PK10

Bottles Nalgene 500 Ml - PK16

2107-0016 Scientific Laboratory Supplies PK16

Bottles Cent Pc 500 Ml - PK4

3140-0500 Scientific Laboratory Supplies PK4

Flat One Piece Moulded Plastic Lid to suit 1 Litre Capacity Bath - EACH

BAT7278 Scientific Laboratory Supplies EACH

Flat One Piece Moulded Plastic Lid to suit 3 Litre Capacity Bath - EACH

BAT7280 Scientific Laboratory Supplies EACH

Flat One Piece Moulded Plastic Lid to suit 6 Litre Capacity Bath - EACH

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Flat One Piece Moulded Plastic Lid to suit 12 Litre Capacity Bath - EACH

BAT7284 Scientific Laboratory Supplies EACH

Flat One Piece Moulded Plastic Lid to suit 30 Litre Capacity Bath - EACH

BAT7286 Scientific Laboratory Supplies EACH

Bottles Amber Hdpe 125 Ml Nn - PK12

2004-0004 Scientific Laboratory Supplies PK12

Adapter 250 mL bottles high-cap - EACH

E5920717006 Scientific Laboratory Supplies EACH

Solution Basin, 55 mL Capacity, White, 50 pieces/CS

42734050-1 Glycomatrix 50 Piece(s)

Bottle Amber Lab Bottles 500ml - PK10

218064406 Scientific Laboratory Supplies PK10

1000 mL Polystyrene Storage Bottles, Neck Size 45 mm, Sterile, 1/pk, 12/cs

344021 NEST Biotechnology 12 pcs/cs

Droso-Filler, Bottles

59-170 Genesee Scientific 1 Filler/Unit

BROWN LOOPED SCREW CAPS WITH "O" RINGS. FITS 0.5ML, 1.5ML AND 2.0ML TUBES

SCO-LP-BR CORNING 500/pk

Bottles Wash W/M Ldpe 500 Ml - PK4

2402-0500 Scientific Laboratory Supplies PK4

Adapter tubes/ bottles/ plates - PK2

E5910751001 Scientific Laboratory Supplies PK2

4oz Clear Bottles - PK80

BP4OZC Scientific Laboratory Supplies PK80

Rack For 9 Bottles 2000ml - EACH

WAS6164 Scientific Laboratory Supplies EACH

Rack For 16 Bottles 1000ml - EACH

WAS6166 Scientific Laboratory Supplies EACH

Bottles N/M Amber 30 Ml Nalgene - PK72

2004-0001 Scientific Laboratory Supplies PK72

Nalgene narrow-mouth boston round bottles HDPE with PP closure bulk pack - PK50

312002-0032 Scientific Laboratory Supplies PK50

FiveGo sample bottles - PK4

PHM0033 Scientific Laboratory Supplies PK4

Rack For 20 Bottles 500ml - EACH

WAS6168 Scientific Laboratory Supplies EACH

Rack For 44 Bottles 100ml - EACH

WAS6172 Scientific Laboratory Supplies EACH

Heat Extractor with Plastic Knob

IMS006 ScyTek Laboratories 1 ea.

Corning Storage Bottles 250ml - PK12

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Corning Storage Bottles 500ml - PK12

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Mycoprep 75Ml Bottles - PK10

240862 Scientific Laboratory Supplies PK10

Honey Jar 375ml with Plastic Cap Sterile - PK100

CON7774 Scientific Laboratory Supplies PK100

Pen for Azlon Bottles - PK2

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Bottles Boston PC 500mL - PK12

BOT0350 Scientific Laboratory Supplies PK12

Bottles Boston PC 1000mL - PK12

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Sample Bottles Undosed Sterile Green Cap 1000ml - PK56

WAT5624 Scientific Laboratory Supplies PK56

Spray bottle with air spray - 50 mL - EACH

DD670885 Scientific Laboratory Supplies EACH

Tube Culture With Svl Plastic - PK40

B99485 Scientific Laboratory Supplies PK40

FoggerJET™ (with plastic Tank set)

PLA005-1NO EWC Diagnostics 1 unit

Pack of 2 suction bottles 100ml - PK2

WAT4874 Scientific Laboratory Supplies PK2

Bottles Powder Clear 250ml - PK42

BP250C Scientific Laboratory Supplies PK42

Powder Bottles 30ml Clear - PK80

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Sample Bottles Undosed Sterile Green Cap 500ml - PK84

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Droso-filler For Bottles - EACH

FLY1282 Scientific Laboratory Supplies EACH

Biological Stain Kit, 5 Bottles

43320000-1 Glycomatrix 1 PK(s)

Reagent Bottles Amber 500ml - PK10

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Staining Dish with Cover Plastic - EACH

X24085 Scientific Laboratory Supplies EACH

Sample Bottles Non Sterile Undosed Red Cap 1000ml - PK56

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500 ml Glass Bottle with cap

B603 ABM Set of 5

Sample Bottles Undosed Sterile Green Cap 250ml - PK140

WAT5604 Scientific Laboratory Supplies PK140

Nalgene Square Media Bottles PETG with HDPE closures in shrink wrapped trays. sterile. - PK280

342020-0030 Scientific Laboratory Supplies PK280

Nalge Nn Bottles ppr 4ltr - PK6

2203-0010 Scientific Laboratory Supplies PK6

Rack For 48 Bottles 50ml - EACH

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Bottles Sod Thio Dosed 18mg/L St. Blue Cap 1000ml - PK56

WAT5620 Scientific Laboratory Supplies PK56

Bottles Sod Thio Dosed 180mg/L St. Blue Cap 1000ml - PK56

WAT5621 Scientific Laboratory Supplies PK56

60ml HDPE Nalgene Bottles - PK1000

3121890002 Scientific Laboratory Supplies PK1000

Sample Bottles EDTA Dosed Sterile White Cap 1000ml - PK56

WAT5622 Scientific Laboratory Supplies PK56

Sample Bottles Non Sterile Undosed Red Cap 500ml - PK84

WAT5616 Scientific Laboratory Supplies PK84

50 ml Tube Storage Boxes, Plastic

B619 ABM 5/pack

Nalgene Bottle Rack for 8x500ml Bottles - EACH

BOT8940 Scientific Laboratory Supplies EACH

M-Vac Jr. PP Barbed Cap for 2L Bottles Red. - EACH

ARG1300 Scientific Laboratory Supplies EACH

Bottles Sod Thio Dosed 18mg/L St. Blue Cap 500ml - PK84

WAT5610 Scientific Laboratory Supplies PK84

Bottles Sod Thio Dosed 180mg/L St. Blue Cap 500ml - PK84

WAT5611 Scientific Laboratory Supplies PK84

250ml Adaptors Conical Bottles - PK2

E5825741005 Scientific Laboratory Supplies PK2

Sample Bottles Non Sterile Undosed Red Cap 250ml - PK140

WAT5606 Scientific Laboratory Supplies PK140

Sample Bottles EDTA Dosed Sterile White Cap 500ml - PK84

WAT5612 Scientific Laboratory Supplies PK84

BottleS Sod. Thio Dosed 18mg/L St. Blue Cap 250ml - PK140

WAT5600 Scientific Laboratory Supplies PK140

Bottles Sod. Thio Dosed 180mg/L St. Blue Cap 250ml - PK140

WAT5601 Scientific Laboratory Supplies PK140

South American FBS Serum 500ml Bottles - 500ML

35-079-CV Scientific Laboratory Supplies 500ML

Sample Bottles EDTA Dosed Sterile White Cap 250ml - PK140

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Boston Bottles 8ml nm HDPE - PK1500

322002-9025 Scientific Laboratory Supplies PK1500

Bottles square narrow PC 2000mL - PK6

BOT0360 Scientific Laboratory Supplies PK6

8-strip Plastic Comb, Compatible with 503711&503761, Sterile, 2/pk, 50/box, 500/

509261 NEST Biotechnology 500 pcs/cs

Amber N/M Bottles Ppr 7.5Ml - PK12

2004-9025 Scientific Laboratory Supplies PK12

Bottles square narrow PC 250mL - PK24

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Bottles square narrow PC 500mL - PK12

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Bottles square narrow PC 1000mL - PK12

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Flystuff (59-194) Droso-Plugs Cap for Bottles - PK1536

FLY1202 Scientific Laboratory Supplies PK1536

Pouring Ring For Simax Bottles - PK10

2070POUR Scientific Laboratory Supplies PK10

8-strip Plastic Comb, Compatible with 503711&503761, Non-Sterile, 2/pk, 50/box,

509211 NEST Biotechnology 500 pcs/cs

Pallet Knife with Plastic Handle 100mm - EACH

SAM1325 Scientific Laboratory Supplies EACH

Pallet Knife with Plastic Handle 200mm - EACH

SAM1326 Scientific Laboratory Supplies EACH

Pallet Knife with Plastic Handle 300mm - EACH

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HDPE Bottles Narrow Mouth 30mL - PK1000

312002-0001 Scientific Laboratory Supplies PK1000

HDPE Bottles Narrow Mouth 60mL - PK1000

312002-0002 Scientific Laboratory Supplies PK1000

Nalgene Rectangular Amber HDPE Bottles 500ml - PK48

2009-0016 Scientific Laboratory Supplies PK48

Wide mouth wash bottles safety labeled

Z423106-1PAK Scientific Laboratory Supplies PK6

Wide mouth wash bottles safety labeled

Z423122-1PAK Scientific Laboratory Supplies PK6

Wide mouth wash bottles safety labeled

Z423149-1PAK Scientific Laboratory Supplies PK6

Wide mouth wash bottles safety labeled

Z423335-1PAK Scientific Laboratory Supplies PK6

Gallium Pieces, 99.99999%

GX9105-10 Glentham Life Sciences 10

Gallium Pieces, 99.99999%

GX9105-100 Glentham Life Sciences 100

Gallium Pieces, 99.99999%

GX9105-50 Glentham Life Sciences 50

Gallium Pieces, 99.9999%

GX1351-10 Glentham Life Sciences 10

Gallium Pieces, 99.9999%

GX1351-100 Glentham Life Sciences 100

Gallium Pieces, 99.9999%

GX1351-50 Glentham Life Sciences 50

Bismuth Pieces, 99.9999%

GX4649-250 Glentham Life Sciences 250

Bismuth Pieces, 99.9999%

GX4649-50 Glentham Life Sciences 50

Antimony Pieces, 99.9999%

GX8733-25 Glentham Life Sciences 25

Antimony Pieces, 99.9999%

GX8733-250 Glentham Life Sciences 250

Antimony Pieces, 99.9999%

GX8733-50 Glentham Life Sciences 50

L.J. Medium Slant in thick glass bottles

SL001T-10SL EWC Diagnostics 1 unit

L.J. Medium Slant in thick glass bottles

SL001T-25SL EWC Diagnostics 1 unit

Support stand with plastic convertor clamp - EACH

HOM2662 Scientific Laboratory Supplies EACH

Stuart Rotisserie for 7x40mm Bottles - EACH

OVE3352 Scientific Laboratory Supplies EACH

Stuart Rotisserie for 2x75mm Bottles - EACH

OVE3354 Scientific Laboratory Supplies EACH

Indium Pieces, 99.999%

GX0999-100 Glentham Life Sciences 100

Indium Pieces, 99.999%

GX0999-25 Glentham Life Sciences 25

Bottles HDPE NM Low Particulate 1L - PK24

BOT1130 Scientific Laboratory Supplies PK24

Plastic tuberculine syringe with drain tube - EACH

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Germanium Pieces max. 2 cm (50 Ohm cm)

GX2202-GX2202100g Glentham Life Sciences GX2202-100g

Germanium Pieces max. 2 cm (50 Ohm cm)

GX2202-GX220210g Glentham Life Sciences GX2202-10g

Germanium Pieces max. 2 cm (50 Ohm cm)

GX2202-GX220250g Glentham Life Sciences GX2202-50g

Bismuth Pieces, 99.999%

GX2132-1 Glentham Life Sciences 1

Bismuth Pieces, 99.999%

GX2132-250 Glentham Life Sciences 250

Bismuth Pieces, 99.999%

GX2132-50 Glentham Life Sciences 50

Rayon Balls X-large For Bottles - PK1000

FLY1270 Scientific Laboratory Supplies PK1000

Drechsel bottles heads Sinter 24/29 - EACH

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Plastic syringe 30ml with tube and clip - EACH

WAT4838 Scientific Laboratory Supplies EACH

Antimony Pieces, 99.999%

GX7845-100 Glentham Life Sciences 100

Antimony Pieces, 99.999%

GX7845-25 Glentham Life Sciences 25

Antimony Pieces, 99.999%

GX7845-500 Glentham Life Sciences 500

Safety Labeled Wash Bottles, Saline Solution

43320027-1 Glycomatrix 1 PK(s)

Bottles HDPE NM Low Particulate 250mL - PK72

BOT1126 Scientific Laboratory Supplies PK72

Bottles HDPE NM Low Particulate 500mL - PK48

BOT1128 Scientific Laboratory Supplies PK48

Gallium Pieces,

GX4776-10 Glentham Life Sciences 10

Gallium Pieces,

GX4776-100 Glentham Life Sciences 100

Gallium Pieces,

GX4776-50 Glentham Life Sciences 50

Wide mouth wash bottles safety labeled - PK6

Z423092-1PAK Scientific Laboratory Supplies PK6

DURAN(R) PRESSURE PLUS BOTTLES VOLUME - PK10

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Duran Stirred Reactor for 250mL Bottles - EACH

BOT1138 Scientific Laboratory Supplies EACH

ELISA Microplate Washer Automated (#MPW-30) bottle set (3 bottles and cap assembly)

MPW-30-SET Alpha Diagnostics 1

Water Sampling Bottles HDPE 500ml Dosed - PK100

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Water Sampling Bottles HDPE 250ml Undosed - PK312

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Water Sampling Bottles HDPE 500ml Undosed - PK100

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Strontium Pieces max. 20 mm, under argon, coated with oil, Ba max. 1%, 99%

GX0588-100 Glentham Life Sciences 100

VOL FLASK,10ML,PLASTIC,CLASS A,PFA,WITH GL-18 SCREW CAP,1/6

5650P-10 CORNING 1/pk

VOL FLASK,100ML,PLASTIC,CLASS A,PFA, WITH GL-18 SCREW CAP,1/6

5650P-100 CORNING 1/pk

VOL FLASK,25ML,PLASTIC,CLASS A,PFA, WITH GL-18 SCREW CAP,1/6

5650P-25 CORNING 1/pk

VOL FLASK,250ML,PLASTIC,CLASS A,PFA, WITH GL-25 SCREW CAP,1/5

5650P-250 CORNING 1/pk

VOL FLASK,50ML,PLASTIC,CLASS A,PFA, WITH GL-18 SCREW CAP,1/6

5650P-50 CORNING 1/pk

VOL FLASK,500ML,PLASTIC,CLASS A,PFA, WITH GL-25 SCREW CAP,1/4

5650P-500 CORNING 1/pk

South American FBS Serum Heat Inactivated 500ml Bottles - 500ML

35-089-CV Scientific Laboratory Supplies 500ML

hybex Media Storage Bottle 2000mL w/ Assorted Caps - PK5

BOT1905 Scientific Laboratory Supplies PK5

hybex Media Storage Bottle 1000mL w/ Assorted Caps - PK10

BOT1904 Scientific Laboratory Supplies PK10

Plungers for the supply/waste bottles x 2 - PK2

WAT4844 Scientific Laboratory Supplies PK2

Gallium Pieces, 99.99%

GX7573-10 Glentham Life Sciences 10

Gallium Pieces, 99.99%

GX7573-100 Glentham Life Sciences 100

Gallium Pieces, 99.99%

GX7573-50 Glentham Life Sciences 50

Holmium Pieces, 99.9%

GX2124-25 Glentham Life Sciences 25

Holmium Pieces, 99.9%

GX2124-5 Glentham Life Sciences 5

Hafnium Pieces, 99.9%

GX4121-10 Glentham Life Sciences 10

Hafnium Pieces, 99.9%

GX4121-100 Glentham Life Sciences 100

Hafnium Pieces, 99.9%

GX4121-25 Glentham Life Sciences 25

Hafnium Pieces, 99.9%

GX4121-250 Glentham Life Sciences 250

Terbium Pieces, 99.9%

GX6615-10 Glentham Life Sciences 10

Terbium Pieces, 99.9%

GX6615-2 Glentham Life Sciences 2

Yttrium pieces, 99.9%

GX0115-10 Glentham Life Sciences 10

Yttrium pieces, 99.9%

GX0115-50 Glentham Life Sciences 50

Thulium Pieces, 99.9%

GX1096-1 Glentham Life Sciences 1

Thulium Pieces, 99.9%

GX1096-5 Glentham Life Sciences 5

Water Sampling Bottles HDPE 250ml Dosed Water Sampling Bottle - PK312

WAT5502 Scientific Laboratory Supplies PK312

Slide Rack (24 Slides), Plastic Basket with Metal Handle

IMS002 ScyTek Laboratories 1 ea.

Lutetium Pieces, 99.99%

GX9538-5 Glentham Life Sciences 5

Samarium Pieces, 99.9%

GX9543-100 Glentham Life Sciences 100

Samarium Pieces, 99.9%

GX9543-25 Glentham Life Sciences 25

Scandium Pieces, 99.9%

GX2769-1 Glentham Life Sciences 1

Scandium Pieces, 99.9%

GX2769-5 Glentham Life Sciences 5

Scandium Pieces, 99.99%

GX0690-1 Glentham Life Sciences 1

Scandium Pieces, 99.99%

GX0690-5 Glentham Life Sciences 5

Lutetium Pieces, 99.9%

GX1068-2 Glentham Life Sciences 2

Lutetium Pieces, 99.9%

GX1068-5 Glentham Life Sciences 5

Ytterbium Pieces, 99.9%

GX9797-25 Glentham Life Sciences 25

Ytterbium Pieces, 99.9%

GX9797-5 Glentham Life Sciences 5

Neodymium Pieces, 99.9%

GX6770-100 Glentham Life Sciences 100

Neodymium Pieces, 99.9%

GX6770-25 Glentham Life Sciences 25

hybex Media Storage Bottle 100mL w/ Assorted Caps - PK10

BOT1901 Scientific Laboratory Supplies PK10

hybex Media Storage Bottle 250mL w/ Assorted Caps - PK10

BOT1902 Scientific Laboratory Supplies PK10

hybex Media Storage Bottle 500mL w/ Assorted Caps - PK10

BOT1903 Scientific Laboratory Supplies PK10

Wash bottle carrier pp holds up to 6 8 oz or 16oz bottles - EACH

Z408514-1EA Scientific Laboratory Supplies EACH

Gadolinium Pieces, 99.9%

GX8860-10 Glentham Life Sciences 10

Gadolinium Pieces, 99.9%

GX8860-50 Glentham Life Sciences 50

50 mL Serological Pipette, Individually Plastic-plastic Warpped, Sterile, 100/pk

329003 NEST Biotechnology 600 pcs/cs

1 L Glass Bottle with cap

B604 ABM Set of 5

2 L Glass Bottle with cap

B605 ABM Set of 5

Bottle 750ml with GL45 Cap - PK10

BOT5215 Scientific Laboratory Supplies PK10

Bottle 150ml with GL45 Cap - PK10

BOT5219 Scientific Laboratory Supplies PK10

Antimony metal pieces

GRM3123-10G EWC Diagnostics 1 unit

250 mL Polystyrene Storage Bottles, Neck Size 45 mm, Sterile, 1/pk, 24/cs

342021 NEST Biotechnology 24 pcs/cs

Further research is needed to better understand spot blotch distribution, susceptibility in winter rye cultivars, and potential yield and quality loss implications in winter rye production and end use. It is unknown how susceptible various winter rye cultivars grown in Kentucky are to spot blotch.

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