Conserved HA-peptide NG34 formulated in pCMV-CTLA4-Ig reduces viral shedding in pigs after a heterosubtypic influenza virus SwH3N2 challenge.

Conserved HA-peptide NG34 formulated in pCMV-CTLA4-Ig reduces viral shedding in pigs after a heterosubtypic influenza virus SwH3N2 challenge.

Swine influenza viruses (SIVs), the causal agents of swine influenza, are not only important to control due to the economic losses in the swine industry, but also can be pandemic pathogens. Vaccination is one of the most relevant strategies to control and prevent influenza infection. Current human vaccines against influenza induce strain-specific immunity and annual update is required due to the virus antigenic shift phenomena. Previously, our group has reported the use of conserved hemagglutinin peptides (HA-peptides) derived from H1-influenza virus as a potential multivalent vaccine candidate.

Immunization of swine with these HA-peptides elicited antibodies that recognized and neutralized heterologous influenza viruses in vitro and demonstrated strong hemagglutination-inhibiting activity. In the present work, we cloned one HA-peptide (named NG34) into a plasmid fused with cytotoxic T lymphocyte-associated antigen (CTLA4) which is a molecule that modifies T cell activation and with an adjuvant activity interfering with the adaptive immune response.

The resulting plasmid, named pCMV-CTLA4-Ig-NG34, was administered twice to animals employing a needle-free delivery approach. Two studies were carried out to test the efficacy of pCMV-CTLA4-Ig-NG34 as a potential swine influenza vaccine, one in seronegative and another in seropositive pigs against SIV. The second one was aimed to evaluate whether pCMV-CTLA4-Ig-NG34 vaccination would overcome maternally derived antibodies (MDA). After immunization, all animals were intranasally challenged with an H3N2 influenza strain.

Treponema p47

trp-243 100µg
EUR 165
Description: Recombinant Treponema pallidum p47

Treponema p15

trp-246 100µg
EUR 165
Description: Recombinant Treponema pallidum p15

Treponema p17

trp-248 100µg
EUR 165
Description: Recombinant Treponema pallidum p17 (Partial)

Treponema p47

trp-249 100µg
EUR 165
Description: Recombinant Treponema pallidum p47 (Partial)

Treponema p15

trp-275 100µg
EUR 165
Description: Recombinant Treponema pallidum p15 (partial)

Treponema TmpA

trp-245 100µg
EUR 165
Description: Recombinant Treponema pallidum TmpA (Partial)

Treponema TmpA

trp-250 100µg
EUR 165
Description: Recombinant Treponema pallidum TmpA

Treponema TP0453

trp-255 100µg
EUR 200
Description: Recombinant Treponema pallidum Outer Membrane Protein TP0453 

Treponema Mosaic

trp-276 100µg
EUR 200
Description: Recombinant Treponema pallidum Mosaic

Treponema pallidum

GWB-E48347 1 ml Ask for price

Treponema pallidum

PCR-H456-PCRH45648D PCR-H456-48D
EUR 230

Treponema pallidum

PCR-H456-PCRH45696D PCR-H456-96D
EUR 312

Treponema pallidum

Oneq-H456-OneqH456100D Oneq-H456-100D
EUR 515

Treponema pallidum

Oneq-H456-OneqH456150D Oneq-H456-150D
EUR 594

Treponema pallidum

Oneq-H456-OneqH45650D Oneq-H456-50D
EUR 413

Treponema p15 His

trp-247 100µg
EUR 165
Description: Recombinant Treponema pallidum p15 (Partial), His Tag

Treponema Pallidum Antibody

abx023643-1mg 1 mg
EUR 718.8

Treponema Pallidum Antibody

abx023644-1mg 1 mg
EUR 718.8

Treponema Pallidum Antibody

abx023677-1mg 1 mg
EUR 718.8

Treponema pallidum Antibody

abx022285-1mg 1 mg
EUR 1195.2

Treponema pallidum Antibody

abx022286-1mg 1 mg
EUR 1195.2

Treponema pallidum Antibody

abx022288-1mg 1 mg
EUR 1195.2

Treponema pallidum Antibody

abx023196-1ml 1 ml
EUR 777.6

Treponema pallidum antibody

10-T77A 1 mg
EUR 429.6
Description: Mouse monoclonal Treponema pallidum antibody

Treponema pallidum antibody

10R-T135A 1 mg
EUR 548.4
Description: Mouse monoclonal Treponema pallidum antibody

Treponema pallidum antibody

20-TG89 1 ml
EUR 125
Description: Goat polyclonal Treponema pallidum antibody

Treponema pallidum antibody

20-TR89 1 mg
EUR 377
Description: Rabbit polyclonal Treponema pallidum antibody

Treponema pallidum antibody

20C-CR1246RP 1 ml
EUR 377
Description: Rabbit polyclonal Treponema pallidum antibody

Treponema pallidum Antibody

GWB-41631C 1 ml Ask for price

Treponema pallidum Antibody

GWB-56D269 1 ml Ask for price

Treponema Pallidum Antibody

GWB-618137 0.2 mg Ask for price

Treponema pallidum Antibody

GWB-62BDEE 1 ml Ask for price

Treponema pallidum, Antibody

GWB-2277A6 1 mg Ask for price

Treponema pallidum Antibody

GWB-16039B 0.1 mg Ask for price

Treponema pallidum Antibody

GWB-850CA9 1 ml Ask for price

Treponema pallidum, Antibody

GWB-93C557 1 mg Ask for price

Treponema pallidum Antibody

GWB-FAFEB9 0.1 mg Ask for price

Treponema Pallidum Antibody

GWB-E73561 1 ml Ask for price

Treponema pallidum Antibody

GWB-C3684E 1 ml Ask for price

Treponema pallidum Antibody

abx022285-400l 400 µl Ask for price

Treponema pallidum Antibody

abx022285-80l 80 µl
EUR 1200

Treponema pallidum Antibody

abx023196-400l 400 µl Ask for price

Treponema pallidum Antibody

abx023196-80l 80 µl
EUR 1150

Treponema p17 16.4kDa

trp-252 100µg
EUR 130
Description: Recombinant Treponema pallidum p17, 16.4 kDa

Treponema p47 47.7kDa

trp-253 100µg
EUR 130
Description: Recombinant Treponema pallidum p47, 47.7kDa

Mouse Anti Treponema Pallidum Monoclonal Antibody

DMABT-51615MT 0.2 mg
EUR 889.2

Treponema p17 22kDa

trp-254 100µg
EUR 165
Description: Recombinant Treponema pallidum p17, 22 kDa

Rabbit Anti Treponema Pallidum Polyclonal Antibody

DPBT-66960RT 1 ml
EUR 920.4

Treponema p41 Mosaic

trp-244 100µg
EUR 165
Description: Recombinant Treponema pallidum p41 Mosaic

Treponema TmpA 45kDa

trp-251 100µg
EUR 130
Description: Recombinant Treponema pallidum TmpA , 45 kDa

Treponema pallidum -HRP

GWB-AC1153 1 ml Ask for price

Treponema pallidum -FITC

GWB-83624F 1 ml Ask for price

Treponema pallidum Antibody (HRP)

abx023195-1ml 1 ml
EUR 777.6

Treponema pallidum antibody (HRP)

60-T69 1 ml
EUR 425
Description: Rabbit polyclonal Treponema pallidum antibody (HRP) conjugated

Treponema pallidum Antibody (HRP)

abx023195-400l 400 µl Ask for price

Treponema pallidum Antibody (HRP)

abx023195-80l 80 µl
EUR 1150

Treponema pallidum Antibody (FITC)

abx023194-1ml 1 ml
EUR 777.6

Treponema pallidum antibody (FITC)

60-T68 1 ml
EUR 425
Description: Rabbit polyclonal Treponema pallidum antibody (FITC) conjugated

Treponema pallidum Antibody (FITC)

abx023194-400l 400 µl Ask for price

Treponema pallidum Antibody (FITC)

abx023194-80l 80 µl
EUR 1150

TREPONEMA PALLIDUM MOSAIC

GWB-96D8B3 1 mg Ask for price

Treponema pallidum protein

30-1287 1 mg
EUR 1195
Description: Purified recombinant Treponema pallidum protein

OAMA02684-1ML - Rabbit anti Treponema pallidum Antibody

OAMA02684-1ML 1ml
EUR 599

Treponema pallidum Antibody (Biotin)

abx023193-1ml 1 ml
EUR 777.6

Treponema pallidum Antibody (Biotin)

GWB-E4BAD4 1 ml Ask for price

Treponema pallidum Antibody (Biotin)

abx023193-400l 400 µl Ask for price

Treponema pallidum Antibody (Biotin)

abx023193-80l 80 µl
EUR 1075

Rabbit Anti Treponema Pallidum Polyclonal Antibody,Biotin

DPBT-66959RT 1 ml
EUR 1057.2

Treponema pallidum PCR kit

PCR-H456-48D 50T
EUR 543.6

Treponema pallidum PCR kit

PCR-H456-96D 100T
EUR 686.4

Treponema phagedenis Treponemal membrane protein B (tmpB)

1-CSB-EP333469THS
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  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Treponema phagedenis Treponemal membrane protein B(tmpB) expressed in E.coli

Treponema Pallidum antibody ELISA Kit

DEIA1013 96T
EUR 639.6
Description: Anti-TP ELISA is an enzyme-linked immunosorbent assay for qualitative determination of antibodies to Treponema Pallidum in human serum or plasma samples. The assay is intended to be used in clinical laboratories for diagnosis and management of patients related to infection with Treponema Pallidum.

Treponema pallidum Ag Protein

abx061388-05mg 0.5 mg
EUR 1328.4

Treponema pallidum p15 Protein

abx060667-1mg 1 mg
EUR 1028.4

Treponema pallidum p15 Protein

abx060668-100ug 100 ug
EUR 878.4

Treponema pallidum p17 Protein

abx060669-1mg 1 mg
EUR 1028.4

Treponema pallidum p17 Protein

abx060670-1mg 1 mg
EUR 1612.8

Treponema pallidum p17 Protein

abx060671-100ug 100 ug
EUR 878.4

Treponema pallidum p41 Protein

abx060672-1mg 1 mg
EUR 1028.4

Treponema pallidum p41 Protein

abx060673-1mg 1 mg
EUR 1696.8

Treponema pallidum p41 Protein

abx060674-1mg 1 mg
EUR 1696.8

Treponema pallidum TMP Protein

abx060676-1mg 1 mg
EUR 1028.4

p17 Treponema pallidum protein

30-1288 1 mg
EUR 1195
Description: Purified recombinant p17 Treponema pallidum protein

p15 Treponema pallidum protein

30-1289 1 mg
EUR 1195
Description: Purified recombinant p15 Treponema pallidum protein

Treponema pallidum p15 protein

30-AT67 500 ug
EUR 1390.8
Description: Purified recombinant Treponema pallidum p15 protein

p47 Treponema Pallidum protein

30-AT71 1 mg
EUR 595
Description: Purified recombinant p47 Treponema Pallidum protein

p17 Treponema Pallidum protein

30-AT72 1 mg
EUR 595
Description: Purified recombinant p17 Treponema Pallidum protein

p15 Treponema Pallidum protein

30-AT75 1 mg
EUR 595
Description: Purified recombinant p15 Treponema Pallidum protein

Treponema pallidum p15 protein

30R-AT065 100 ug
EUR 295
Description: Purified recombinant Treponema pallidum p15 protein

Treponema pallidum p17 protein

30R-AT066 500 ug
EUR 867
Description: Purified recombinant Treponema pallidum p17 protein

Treponema pallidum p47 protein

30R-AT067 500 ug
EUR 875
Description: Purified recombinant Treponema pallidum p47 protein

Treponema pallidum p17 Protein

20-abx260170
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  • 0.5 mg
  • 100 ug
  • 1 mg

Treponema pallidum p41 Protein

20-abx260171
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  • 0.5 mg
  • 100 ug
  • 1 mg

Treponema pallidum p47 Protein

20-abx260172
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  • 0.5 mg
  • 100 ug
  • 1 mg

Treponema pallidum p15 Protein

20-abx260316
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  • Ask for price
  • 0.5 mg
  • 100 ug
  • 1 mg

p47 Treponema Pallidum protein

80-1395 1 mg
EUR 136
Description: Purified recombinant p47 Treponema Pallidum protein

Treponema Pallidum protein (HRP)

80R-2409 1 ml
EUR 500
Description: Purified recombinant Treponema pallidum protein

Treponema pallidum p15 Protein

abx060667-100g 100 µg Ask for price

Treponema pallidum p15 Protein

abx060667-10g 10 µg
EUR 1500

Treponema pallidum p15 Protein

abx060667-50g 50 µg Ask for price

Treponema pallidum p15 Protein

abx060668-100g 100 µg Ask for price

Treponema pallidum p15 Protein

abx060668-10g 10 µg
EUR 800

Treponema pallidum p15 Protein

abx060668-50g 50 µg Ask for price

Treponema pallidum p17 Protein

abx060669-100g 100 µg Ask for price

Treponema pallidum p17 Protein

abx060669-10g 10 µg
EUR 1500

Treponema pallidum p17 Protein

abx060669-50g 50 µg Ask for price

Treponema pallidum p17 Protein

abx060670-100g 100 µg Ask for price

Treponema pallidum p17 Protein

abx060670-10g 10 µg
EUR 1625

Treponema pallidum p17 Protein

abx060670-50g 50 µg Ask for price

Treponema pallidum p17 Protein

abx060671-100g 100 µg Ask for price

Treponema pallidum p17 Protein

abx060671-200g 200 µg Ask for price

Treponema pallidum p17 Protein

abx060671-50g 50 µg
EUR 800

Treponema pallidum p41 Protein

abx060672-100g 100 µg Ask for price

Treponema pallidum p41 Protein

abx060672-10g 10 µg
EUR 1500

Treponema pallidum p41 Protein

abx060672-50g 50 µg Ask for price

Treponema pallidum p41 Protein

abx060673-100g 100 µg Ask for price

Treponema pallidum p41 Protein

abx060673-10g 10 µg
EUR 1575

Treponema pallidum p41 Protein

abx060673-50g 50 µg Ask for price

Treponema pallidum p41 Protein

abx060674-100g 100 µg Ask for price

Treponema pallidum p41 Protein

abx060674-10g 10 µg
EUR 1575

Treponema pallidum p41 Protein

abx060674-50g 50 µg Ask for price

Treponema pallidum TMP Protein

abx060676-100g 100 µg Ask for price

Treponema pallidum TMP Protein

abx060676-10g 10 µg
EUR 1500

Treponema pallidum TMP Protein

abx060676-50g 50 µg Ask for price

Treponema pallidum p17 Protein

abx260170-20g 20 µg
EUR 950

Treponema pallidum p17 Protein

abx260170-5g 5 µg
EUR 350

Treponema pallidum p41 Protein

abx260171-20g 20 µg
EUR 950

Treponema pallidum p41 Protein

abx260171-5g 5 µg
EUR 350

Treponema pallidum p47 Protein

abx260172-20g 20 µg
EUR 950

Treponema pallidum p47 Protein

abx260172-5g 5 µg
EUR 350

Treponema pallidum p15 Protein

abx260316-10g 10 µg
EUR 950

Treponema pallidum p15 Protein

abx260316-2g 2 µg
EUR 350

Treponema pallidum RT PCR kit

RTq-H456-100D 100T
EUR 860.4

Treponema pallidum RT PCR kit

RTq-H456-150D 150T
EUR 969.6

Treponema pallidum RT PCR kit

RTq-H456-50D 50T
EUR 717.6

Treponema pallidum (T. pallidum) Antibody

abx411659-1ml 1 ml
EUR 610.8

Treponema pallidum (T. pallidum) Antibody

abx411659-200g 200 µg
EUR 425

Treponema pallidum TmpA Protein

abx060675-100ug 100 ug
EUR 878.4

Treponema pallidum TmpA protein

30R-AT068 100 ug
EUR 292
Description: Purified recombinant Treponema pallidum TmpA protein

Treponema pallidum TmpA Protein

20-abx260320
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  • 0.5 mg
  • 100 ug
  • 1 mg

Treponema pallidum (Syphilis) IgG

DE3517 96
EUR 98

Treponema pallidum (Syphilis) IgM

DE4267 96
EUR 98

Treponema pallidum TmpA protein

80-1396 1 mg
EUR 150
Description: Purified recombinant Treponema pallidum TmpA protein

Treponema pallidum TmpA Protein

abx060675-100g 100 µg Ask for price

Treponema pallidum TmpA Protein

abx060675-10g 10 µg
EUR 800

Treponema pallidum TmpA Protein

abx060675-50g 50 µg Ask for price

Treponema pallidum TmpA Protein

abx260320-10g 10 µg
EUR 950

Treponema pallidum TmpA Protein

abx260320-2g 2 µg
EUR 350

p47 Treponema pallidum (His tag)

80-1371 1 mg
EUR 1125
Description: Purified recombinant p47 Treponema pallidum (His tag)

Treponema pallidum (Syphilis) Antibody (p47)

GWB-T00691 100ug Ask for price

Treponema pallidum (Syphilis) Antibody (p47)

GWB-T00693 100ug Ask for price

QPCR Kit DNA Treponema pallidum

MOL8366 EACH
EUR 1031.58

Treponema Pallidum IgG ELISA Kit

E4676-100 each
EUR 646.8

Treponema Pallidum IgM ELISA Kit

E4677-100 each
EUR 646.8

QPCR Kit DNA Treponema denticola

MOL8352 EACH
EUR 772.92

QPCR Kit DNA Treponema denticola

MOL8354 EACH
EUR 982.68

Treponema pallidum p15 recombinant antigen

8945 100 ug
EUR 477.6
Description: This is Treponema pallidum p15 recombinant antigen for ELISA,WB.

Treponema pallidum p17 recombinant antigen

8946 100 ug
EUR 477.6
Description: This is Treponema pallidum p17 recombinant antigen for ELISA,WB.

Treponema pallidum p47 recombinant antigen

8947 100 ug
EUR 477.6
Description: This is Treponema pallidum p47 recombinant antigen for ELISA,WB.

Treponema pallidum p17 (Recombinant)

22060731-1 100 µg
EUR 264.47

Treponema pallidum p41 (Recombinant)

22060732-1 100 µg
EUR 264.47

Treponema pallidum p47 (Recombinant)

22060733-1 100 µg
EUR 264.47

Recombinant Treponema pallidum p15

7-07555 100µg Ask for price

Recombinant Treponema pallidum p15

7-07556 500µg Ask for price

Recombinant Treponema pallidum p15

7-07557 1000µg Ask for price

Recombinant Treponema pallidum p17

7-07561 100µg Ask for price

Recombinant Treponema pallidum p17

7-07562 500µg Ask for price

Recombinant Treponema pallidum p17

7-07563 1000µg Ask for price

Recombinant Treponema pallidum p41

7-07567 100µg Ask for price

Recombinant Treponema pallidum p41

7-07568 500µg Ask for price

Recombinant Treponema pallidum p41

7-07569 1000µg Ask for price

Recombinant Treponema pallidum p47

7-07573 100µg Ask for price

Recombinant Treponema pallidum p47

7-07574 500µg Ask for price

Recombinant Treponema pallidum p47

7-07575 1000µg Ask for price

Treponema pallidum p17 Recombinant

rAP-5486 Inquiry Ask for price

Treponema pallidum p41 Recombinant

rAP-5487 Inquiry Ask for price

Treponema pallidum p47 Recombinant

rAP-5488 Inquiry Ask for price

Treponema pallidum p15 Recombinant

rAP-5491 Inquiry Ask for price

Mouse antibody for Treponema pallidum p47

2126 100 ug
EUR 386.26
Description: This is purified Mouse monoclonal antibody against Treponema pallidum p47 for WB, ELISA.

Mouse antibody for Treponema pallidum p47

2128 100 ug
EUR 386.26
Description: This is purified Mouse monoclonal antibody against Treponema pallidum p47 for WB, ELISA.

Treponema pallidum TmpA recombinant antigen

8948 100 ug
EUR 477.6
Description: This is Treponema pallidum TmpA recombinant antigen for ELISA,WB.

OAMA00328-1MG - Treponema pallidum Antibody

OAMA00328-1MG 1mg
EUR 949

Recombinant Treponema pallidum TmpA

7-07582 100µg Ask for price

A complete elimination or significant reduction in the viral shedding was observed within the first week after the challenge in the vaccinated animals from both studies. In addition, no challenged heterologous virus load was detected in the airways of vaccinated pigs. Overall, it is suggested that the pCMV-CTLA4-Ig-NG34 vaccine formulation could potentially be used as a multivalent vaccine against influenza viruses.

Conserved HA-peptide NG34 formulated in pCMV-CTLA4-Ig reduces viral shedding in pigs after a heterosubtypic influenza virus SwH3N2 challenge.
Conserved HA-peptide NG34 formulated in pCMV-CTLA4-Ig reduces viral shedding in pigs after a heterosubtypic influenza virus SwH3N2 challenge.

Sowing the Seeds of a Pandemic? Mammalian Pathogenicity and Transmissibility of H1 Variant Influenza Viruses from the Swine Reservoir.

Emergence of genetically and antigenically diverse strains of influenza to which the human population has no or limited immunity necessitates continuous risk assessments to determine the likelihood of these viruses acquiring adaptations that facilitate sustained human-to-human transmission.

As the North American swine H1 virus population has diversified over the last century by means of both antigenic drift and shift, in vivo assessments to study multifactorial traits like mammalian pathogenicity and transmissibility of these emerging influenza viruses are critical. In this review, we examine genetic, molecular, and pathogenicity and transmissibility data from a panel of contemporary North American H1 subtype swine-origin viruses isolated from humans, as compared to H1N1 seasonal and pandemic viruses, including the reconstructed 1918 virus.

We present side-by-side analyses of experiments performed in the mouse and ferret models using consistent experimental protocols to facilitate enhanced interpretation of in vivo data. Contextualizing these analyses in a broader context permits a greater appreciation of the role that in vivo risk assessment experiments play in pandemic preparedness. Collectively, we find that despite strain-specific heterogeneity among swine-origin H1 viruses, contemporary swine viruses isolated from humans possess many attributes shared by prior pandemic strains, warranting heightened surveillance and evaluation of these zoonotic viruses.

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